Toyobo is a leading manufacturer of enzymes and reagents for life science research and industrial fields based on our original technologies about PCR enzymes. It consists of specially engineered ezymes with high efficiency according to various unique PCR enzymes, kits and PCR related products as shown below:
Enzyme | Product Name | Application | PCR Product Ends |
High fidelity DNA polymerase | KOD -Plus- Neo | Cloning | blunt |
KOD FX Neo | - Genotyping - Cloning high G/C*applicable with crude sample | blunt | |
KOD Dash | - Colony PCR - Detection | mixed (blunt & 3'-dA) | |
KOD -Plus- Mutagenesis Kit | - | ||
Tth DNA polymerase | rTth DNA Polymerase | - General PCR - RT-PCR | 3'-dA |
RT-PCR Quick Master Mix | One-step RT-PCR | 3'-dA | |
Taq DNA polymerase | Quick Taq® HS DyeMix | - Colony PCR - General PCR | 3'-dA |
Blend Taq® -Plus- | - General PCR (longer target) - Colony PCR - Detection | mixed (blunt & 3'-dA) |
cDNA synthesis Kits
Toyobo has various kits for cDNA synthesis based on the genetically improved M-MLV reverse transcriptase "ReverTra Ace®". ReverTra Ace -α-® is an efficient and convenient kit to synthesize high quality cDNA. This kit contains the highly efficient reverse transcriptase "ReverTra Ace®", as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR.
Product Name | Application | Long cDNA synthesis | Master Mix | Recommend PCR enzymes |
ReverTra Ace -α-® | Conventional RT-PCR | ++ (> 10 kb) | KOD –Plus- series KOD FX series Taq-based PCR reagent | |
ReverTra Ace® qPCR RT Kit | qPCR | + (< 5 kb) | THUNDERBIRD®series Realtime PCR Master Mix series KOD SYBR® qPCR Mix KOD –Plus- series KOD FX series Taq-based PCR reagent | |
ReverTra Ace® qPCR RT Master Mix | qPCR | + (< 5 kb) | ||
ReverTra Ace® qPCR RT Master Mix with gDNA Remover | qPCR | + (< 5 kb) | ||
SuperPrep® Cell Lysis & RT Kit for qPCR | qPCR from cultured cells | + (< 5 kb) |